This is a knockout-validated antibody summary, based on the publication "Caspase-8 scaffolding function and MLKL regulate NLRP3 inflammasome activation downstream of TLR3", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblots of RIPK3 of macrophages derived from WT (n=3) or RIPK3-KO (n=3) mice. Caspase-1 is used as loading control. From [1].

Antibody: RIPK3

Catalog number: R4277

Summary: Rabbit polyclonal against a synthetic peptide corresponding to amino acids 473-486 of murine RIP3. It reacts with rat, mouse, human in western blot.

Company: Sigma-Aldrich, now a part of MilliporeSigma

Western blot

Macrophages derived from WT (n=3) or RIPK3-KO (n=3) mice

1/1,000–1/2,000 dilution

Caspase-1 might not be a good housekeeping gene to use as a loading control, but the authors concluded that it showed no changes.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.