This is a knockout-validated antibody summary, based on the publication "The necroptosis-inducing kinase RIPK3 dampens adipose tissue inflammation and glucose intolerance", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot analysis of RIPK3 in epididymal white adipose tissue from WT and RIPK3-/- mice fed with normal chow diet (-) or a choline-deficient high-fat diet (CD-HFD) for 16 weeks. GAPDH was used as loading control. From [1].

Rabbit polyclonal IgG

Company: IMGENEX

Antibody: RIPK3

Catalog number: NBP1-77299

Summary: Rabbit polyclonal IgG against a peptide corresponding to amino acids 473 to 486 of murine RIP3. Reacts with mouse, human and rat. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin), and ELISA.

Western blot

Epididymal white adipose tissue from WT and RIPK3-/- mice. Tissue samples were homogenized in NP-40 lysis buffer using a tissue grind pestle (Kimble/Chase) or with a bead ruptor 12 (Omni International) to obtain protein lysates.

1:2,000 dilution.

1:5,000 dilution anti-rabbit-horseradish peroxidase (#NA934V).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.