This is a knockout-validated antibody summary, based on the publication "LRRK2 enhances Nod1/2-mediated inflammatory cytokine production by promoting Rip2 phosphorylation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblotting of Rip2 in WT, Nod2−/−, and Rip2−/− murine BMDMs treated with 5 ng/mL LPS and 10 μg/mL MDP for 30 minutes or mock-treated. Actin was used as a loading control. From [1].

Mouse monoclonal IgG2a Kappa

Company: Abnova

Antibody: RIPK2

Catalog number: H00008767-M02

Summary: Mouse monoclonal IgG2a Kappa raised against a partial recombinant RIPK2 (a.a. 431- 540). Reacts with human, mouse and rat. Suitable for western blot, immunohistochemistry (paraffin), and ELISA.

Western blot

WT, Nod2−/−, and Rip2−/− murine bone marrow-derived macrophages.

Anti-LRRK2 antibody from Epitomics (MJFF2 c41-2) is also KO validated (Figure 5B).

The same clone (6F7) is sold as LifeSpan Biosciences LS-C133569; Abnova H00008767-M02; Sigma-Aldrich SAB1404621.