This is a knockout-validated antibody summary, based on publications "Melatonin attenuates chronic pain related myocardial ischemic susceptibility through inhibiting RIP3-MLKL/CaMKII dependent necroptosis", as cited below [1], and "Microbial signals, MyD88, and lymphotoxin drive TNF-independent intestinal epithelial tissue damage" for western blot knockout validation (figure 6d) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Cell Signaling
Antibody: RIP3
Catalog number: 95702
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Val370 of mouse RIP3 protein. Reacts with mouse by western blot, immunoprecipitation, immunofluorescence (immunocytochemistry) and flow cytometry.
Western blot
Heart tissue from wild-type and RIP3 KO mice.
3% nonfat milk in PBST for 1 h.
At 4 °C over the night.
1:5,000 dilution horseradish peroxidase-conjugated antibodies for 1 h at room temperature.
Chemiluminescence reagents (Millipore, Billerica, MA, USA) was used to visualize the blots.
Representative western blots for myocardial RIP3 expression in RIP3 knockout (RIP3 KO) mice and their wild-type (WT) littermates. Please see Figure 3b in the article [1].
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