RIP3 antibody | knockout validation | Cell Signaling 13526

This is a knockout-validated antibody summary, based on the publication "ICP6 Prevents RIP1 Activation to Hinder Necroptosis Signaling", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

RIP3 antibody | knockout validation | Cell Signaling 13526 figure 1
Figure 1. WT and RIP3-KO HT29 cells with indicated lentivirus infection were treated with T/S/Z for indicated time. Cells were immunoblotted for the indicated proteins. β-Actin and total RIP1 were shown as loading control. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: RIP3

Catalog number: 13526

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues near the carboxy terminus of human RIP3 protein. Reacts with human. Suitable for western blot and immunoprecipitation.

Validation Method

Western blot

Sample

WT and RIP3-KO HT29 cells.

References
  1. Hu H, Wu G, Shu Z, Yu D, Nan N, Yuan F, et al. ICP6 Prevents RIP1 Activation to Hinder Necroptosis Signaling. Front Cell Dev Biol. 2020;8:595253 pubmed publisher