This is a knockout-validated antibody summary, based on the publication "Nuclear TRIM25 Specifically Targets Influenza Virus Ribonucleoproteins to Block the Onset of RNA Chain Elongation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunoblot of the indicated proteins in WT, RIG-I knockout, and RIG-I/TRIM25 (T25) double-knockout human A549 cells 24 h after infection with Udorn virus. Please see Figure 2a in the article [1].

Mouse monoclonal IgG1λ

Company: Millipore

Antibody: RIG-I

Catalog number: MABF297

Summary: Mouse monoclonal IgG1λ against recombinant protein corresponding to human RIG-I. Reacts with human by western blot, immunoprecipitation and immunocytochemistry.

Western blot

WT, RIG-I knockout, and RIG-I/TRIM25 double-knockout human A549 cells. Cells were lysed in 150 mM NaCl, 50 mM Tris-HCl (pH 7.4), 1% NP-40, 2.5 mM MgCl2, 5 mM EDTA, 10% glycerol, and supplemented with Complete protease inhibitor (Roche, #11836170001).

TBST (0.1%) containing 3% nonfat dry milk for 30 minutes at room temperature.

1:1,000 dilution diluted in TBST (0.1%) for 1 hr at room temperature.

1:10,000 dilution donkey anti-mouse horseradish peroxidase-conjugated (Thermo Scientific, #32430) diluted in TBST (0.1%) containing 3% nonfat dry milk for 40 minutes at room temperature.

ECL Plus detection reagent (GE Healthcare, #RPN2132).