This is a knockout-validated antibody summary, based on the publication "The Human Papillomavirus E6 Oncoprotein Targets USP15 and TRIM25 To Suppress RIG-I-Mediated Innate Immune Signaling", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1
Company: Adipogen
Antibody: RIG-I
Catalog number: AG-20B-0009-C100
Summary: Mouse monoclonal IgG1 against recombinant human RIG-I (aa 201-713). Reacts with human and mouse. Suitable for western blot, immunohistochemistry (paraffin), and immunoprecipitation.
Western blot
Control and RIG-I KO HaCaT cells.
5% (wt/vol) nonfat dry milk in PBS-Tween 20 for 1 h.
1:1,000 dilution either 1 h at room temperature or at 4°C overnight.
1:2,500 dilution secondary anti-mouse IgG-horseradish peroxidase (7076S; Cell Signaling) antibodies at room temperature for 1 h.
Protein bands were visualized with the enhanced SuperSignal West Pico chemiluminescence reagent (Thermo Fisher) and were detected by a luminescent imaging system (LAS-4000; Fuji).
Western blot of HaCaT cells expressing nontargeting control guide RNA (NT) or transduced with guide RNA targeting RIG-I (RIG-I KO). Actin served as a loading control. Please see Figure 5b in the article [1].
The same clone (Alme-1) is sold as LifeSpan Biosciences LS-C344741, LS-C344928; Abnova MAB16084; Adipogen AG-20B-0009-C100.
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