This is a knockout-validated antibody summary, based on the publication "A G-quadruplex DNA structure resolvase, RHAU, is essential for spermatogonia differentiation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit polyclonal
Company: Protein Technologies
Antibody: RHAU
Catalog number: 13159-1-AP
Summary: Rabbit polyclonal against human DHX36 fusion protein. Reacts with human, mouse, and rat. Recommended for western blot, immunohistochemistry, immunoprecipitation and ELISA.
Western blot
Testes from P35 control (fl/fl) and RHAU deletion (fl/fl: Cre) mice. Testes were ground on ice in the lysis buffer (1% NP-40, 1% sodium deoxycholate, 0.1% SDS, 150 mM sodium chloride, 2 mM EDTA, 10 mM sodium phosphate, pH 7.2), which was supplemented with protease inhibitor cocktail (Roche, Shanghai, China) and 1 mM phenylmethylsulfonyl fluoride.
1:100 dilution.
ECL chemiluminescence (GE Healthcare, Pittsburgh, PA, USA).
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.