This is a knockout-validated antibody summary, based on the publication "Regulation of energy homeostasis by the ubiquitin-independent REGγ proteasome", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of REGγ in MEF cells from wild-type (+/+, WT) and REGγ knockout (−/−, KO) mice. Actin was used as loading control. From [1].

Mouse monoclonal IgG1

Company: BD

Antibody: REGgamma

Catalog number: 611181

Summary: Mouse monoclonal IgG1 against mouse Psme3/PA28-γ/REG-γ aa. 45-147. Reacts with mouse, rat, human and dog. Suitable for western blot and immunofluorescence.

Western blot

MEF cells from wild-type and REGγ knockout mice. Cells were lysed in lysis buffer (50 mM Tris-HCl pH 8.0, 5 mM EDTA, 150 mM NaCl, 0.5% NP-40, 1 mM PMSF).

Phosphate-buffered saline (PBS) containing 5% nonfat dry milk and 0.1% Tween 20 for 1 h.

1/2,000 dilution.

HRP-conjugated secondary antibodies.

ECL-plus reagent (Amersham).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.