This is a knockout-validated antibody summary, based on the publication "Ubiquitin-independent degradation of cell-cycle inhibitors by the REGgamma proteasome", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit polyclonal
Company: Zymed (now Thermo Fisher Scientific)
Antibody: REG-gamma (PA28-gamma)
Catalog number: 38-3800
Summary: Rabbit polyclonal antibody raised against a synthetic peptide corresponding to the N-terminal portion of mouse, rat and pig PA28-gamma. Supplier recommended for IP, WB and flow cytometry. Reacts with human, mouse, primate and rat PA28-gamma. Antibody is antigen affinity purified.
Western blot
Lysates of REG-gamma +/- and REG-gamma -/- murine embryonic fibroblasts.
Anti-REG-gamma
HRP-conjugated anti-rabbit IgG (Amersham Lifesciences).
See figure 4 from article [1].
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.
- Chen X, Barton L, Chi Y, Clurman B, Roberts J. Ubiquitin-independent degradation of cell-cycle inhibitors by the REGgamma proteasome. Mol Cell. 2007;26:843-52 pubmed
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