This is a knockout-validated antibody summary, based on the publication "Validation of a commercially available anti-REDD1 antibody using RNA interference and REDD1-/- mouse embryonic fibroblasts", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit polyclonal
Company: Proteintech
Antibody: REDD1
Catalog number: 10638-1-AP
Summary: Rabbit polyclonal antibody raised against an E.coli-expressed fusion protein containing residues 1-232 of human REDD1.
Supplier recommended for WB, IP, IF, IHC and elisa.
Antigen affinity-purified.
Reacts with human, mouse, rat, gerbil, pig, rabbit and Meriones unguiculatus REDD1.
Western blot
Lysates of wild-type and REDD1 -/- mouse embryonic fibroblasts.
TBS (20mM Tris-base, 150mM NaCl, 50mM KCl) containing 5% milk powder and 0.2% Tween 20.
Anti-REDD1 antibody diluted 1:800 in TBS containing 1% milk powder and 0.2% Tween 20. Overnight incubation.
HRP-conjugated Affinity Pure goat anti-rabbit IgG (Jaclson ImmunoResearch Laboratories) diluted 1:7500 in blocking buffer.
Clarity Western ECL Blotting reagent (BioRad).
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.
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