This is a knockout-validated antibody summary, based on the publication "Rbpj direct regulation of Atoh7 transcription in the embryonic mouse retina", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of E14.5 lens protein extracts, collected from RbpjCKO/CKO, Le-Cre;RbpjCKO/+ and Le-Cre;RbpjCKO/CKO embryos, respectively. A single band of 56 kDa, the predicted size of Rbpj, is absent from mutant lenses, with β-actin loading control at bottom. From [1].

Rat monoclonal IgG1

Company: CosmoBio

Antibody: RBP-Jk

Catalog number: SIM-2ZRBP1

Summary: Rat monoclonal IgG1 against mouse RBP-Jk. Reacts with mouse by western blot, immunohistochemistry, immunoprecipitation, chromatin IP and ELISA.

Western blot

Eye lenses from control and Rbpj-KO mouse embryos. Lenses were lysed in RIPA buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 0.5% sodium deoxycholate, 0.1% SDS, 1% NP40) containing Complete protease inhibitors (Sigma 11697498001).

1:500 dilution.

1:5,000 dilution HRP-conjugated anti-rat IgG secondary antibodies (Jackson Immunoresearch, 112-035-175).

Supersignal West Pico Chemiluminescent substrate kit (Thermo Scientific, 34078), Kodak standard x-ray film and film developer.