This is a knockout-validated antibody summary, based on the publication "The small G-protein RalA promotes progression and metastasis of triple-negative breast cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Representative images of RALA and RALB immunofluorescence staining in MDA-MB-231 Ctrl, RALA-KO and RALB-KO cells (red = RALA or RALB, blue = DAPI; scale bars = 20μm). From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: RALA

Catalog number: ab126627

Summary: Rabbit monoclonal antibody against a synthetic peptide within human RALA (aa 100-200). Reacts with human. Suitable for western blot, immunohistochemistry (paraffin) and flow cytometry. Unsuitable for immunoprecipitation or immunocytochemistry/immunofluorescence.

Immunocytochemistry

Control and RALA-KO MDA-MB-231 cells.

5% BSA-0.3% Triton x-100 solution.

1:200 dilution in blocking buffer overnight at 4°C.

1:250 dilution AlexaFluor 594 anti-rabbit secondary antibody (Invitrogen, A21207) for 1h at room temperature.

Zeiss LSM 800 confocal microscope.