This is a knockout-validated antibody summary, based on the publication "Epidermal RAF prevents allergic skin disease", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. RAF1 and BRAF in wild-type and conditionally double knockout epidermal cells from 3 weeks old F/F2 and Δ/Δep2 (n = 4). ACTB is shown as a loading control, from figure 1 [1].

Company: Santa Cruz

Antibody: RAF1

Catalog number: sc-133

Summary:: Raf-1 Antibody (C-12) is a rabbit polyclonal IgG raised against epitope mapping at the C-terminus of Raf-1 of human origin, recommended for detection of Raf-1 p74 of mouse, rat and human origin by WB, IP, IF, KA and ELISA; also reactive with additional species, including equine, canine and bovine

Western blot

Epidermis was lysed with Laemmli buffer at 95°C for 5 minutes.

Lysates were immunoblotted at 1:1000 dilution.