RAF1 antibody | knockout validation | Santa Cruz Biotechnology sc-133
DOI
//dx.doi.org/10.13070/ko.en.6.1930
Date
2016-12-18

This is a knockout-validated antibody summary, based on the publication " Epidermal RAF prevents allergic skin disease", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

RAF1 antibody | knockout validation | Santa Cruz Biotechnology sc-133 figure 1
Figure 1. From Figure 6 in [1]. Western blot of lysates of control epidermal cells and epidermal cells with RAF1 knockout or RAF1/BRAF double knockout. Probed with anti-RAF1 antibody. ACTB serves as a loading control.
Antibody information

Rabbit polyclonal

Company: Santa Cruz Biotechnology

Antibody: RAF1

Catalog number: sc-133

Summary: Rabbit polyclonal antibody raised against an epitope within the C-terminal region of human RAF1.

Supplier recommended for WB, IP,IF, kinase assay and elisa.

Reacts with human, mouse and rat RAF1.

Validation Method

Western blot

Sample

Lysates of control epidermal cells and epidermal cells with RAF1 knockout or RAF1/BRAF double knockout.

Primary incubation

Anti-RAF1 antibody.

Disclaimer

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

References
  1. Raguz J, Jerić I, Niault T, Nowacka J, Kuzet S, Rupp C, et al. Epidermal RAF prevents allergic skin disease. elife. 2016;5: pubmed publisher