This is a knockout-validated antibody summary, based on the publication "Trans-kingdom mimicry underlies ribosome customization by a poxvirus kinase", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Cell Signaling
Antibody: RACK1
Catalog number: 5432
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to central residues of human RACK1 protein. Reacts with human, mouse, rat, monkey, and D. melanogaster. Suitable for western blot and immunoprecipitation.
Western blot
Wild type or RACK1-knockouts (KO1, KO2). Whole-cell lysates were prepared in lysis buffer (62.5mM Tris-HCl at pH 6.8, 2% SDS, 10% glycerol, 0.7M β-mercaptoethanol).
5% non-fat dry milk, 0.1% Tween in TBS for 1h at room temperature.
Diluted in 3% BSA, 0.1% Tween in TBS overnight at 4°C.
1:3,000 dilution HRP-conjugated secondary antibody (GE Healthcare Life Sciences) in 5% non-fat dry milk, 0.1% tween in TBS for 1h at room temperature.
Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific) for 1min before exposure to X-ray film.
Western blot analysis of samples from wild type or RACK1-knockouts (KO1, KO2). Please see Figure 1b in the article [1].
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