This is a knockout-validated antibody summary, based on the publication "Chloroquine-Inducible Par-4 Secretion Is Essential for Tumor Cell Apoptosis and Inhibition of Metastasis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Rab8 wild-type (WT), Rab8b−/−, or Rab8a−/− MEFs were treated with chloroquine (CQ, 25 µM) or vehicle (V) for 24 hr, and the lysates were examined by western blot analysis with the indicated antibodies. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: RAB8A

Catalog number: ab188574

Summary: Rabbit monoclonal IgG against a synthetic peptide within human RAB8A (aa 150 to the C-terminus). Reacts with mouse, rat, and human. Suitable for western blot, immunohistochemistry (paraffin), immunocytochemistry/immunofluorescence, and flow cytometry.

Western blot

Rab8 wild-type (WT), Rab8b−/−, or Rab8a−/− MEFs.

Par-4 (Santa Cruz, R334), Rab8b (Abcam, ab124356) and p53 (1C12, Cell Signaling) are also KO validated by WB and IC (RAB8B).