This is a knockout-validated antibody summary, based on the publication "Reciprocal effects of rab7 deletion in activated and neglected T cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: RAB7

Catalog number: 9367

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Glu188 of human Rab7 protein. Reacts with human, mouse, rat, and monkey. Suitable for western blot, immunoprecipitation, and immunofluorescence/immunocytochemistry.

Western blot | Immunocytochemistry

Rab7+/+ and rab7−/− MEFs. WB: cells were lysed in the plate on ice with lysis buffer (2% Triton X-100, 80 mM HEPES, 0.4 mM EDTA, pH 6.6) containing complete protease inhibitor cocktail (Roche), 50 mM sodium fluoride, 100 nM sodium orthovanadate, and 50 mM β-glycerophosphate.

ICC: PBS containing 0.3% saponin, 10% FBS and 0.05% NaN3.

ICC: 1:100 dilution in blocking buffer overnight at 4°C.

WB: secondary goat anti-rabbit (LI-COR, 92632211).

ICC: 1:100 dilution secondary anti-rabbit Alexa488 in blocking buffer for 2 h at RT.

EB: blots were scanned with an Odyssey imager (LI-COR Biosciences).

ICC: Nikon Eclipse TE2000 fluorescence microscope equipped with a CoolSNAP CCD camera (Photometrics).

Please see Figure 1c,e in the article [1].

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.