This is a knockout-validated antibody summary, based on the publication "PDHA1 Gene Knockout In Human Esophageal Squamous Cancer Cells Resulted In Greater Warburg Effect And Aggressive Features In Vitro And In Vivo", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

[enlarge]

Figure 1. Immunocytochemical analysis of PDHA1 expression, where PDHA1 protein expression in the PDHA1 KO cells is negative while its expression in the control cells is positive. From [1].

Company: Abcam

Antibody: Pyruvate Dehydrogenase E1-alpha subunit (phospho S293)

Catalog number: ab177461

Summary: Rabbit monoclonal IgG against a synthetic peptide within human Pyruvate Dehydrogenase E1-alpha subunit aa 250-350 (Cysteine residue). Reacts with mouse, rat and human. Suitable for western blot, immunohistochemistry (paraffin), immunoprecipitation and ELISA.

[enlarge]

Figure 2. WB analysis of PDHA1 expression, where PDHA1 protein expression in the PDHA1 KO cells is negative while its expression in the control cells is positive. From [1].

Western blot | Immunocytochemistry

WT and PDHA1 KO KYSE450 cells.

WB: 5% milk (1 hr, room temperature).

ICC: 3% H2O2 (25 mins) and 3% BSA (30 mins).

WB: overnight at 4°C.

ICC: 1:100 dilution at 4°C, overnight.

WB: horseradish peroxidase conjugated (1 hr, room temperature).

WB: photographic developer (Western Dura Extended Duration Substrate, SuperSignal, USA) and X-ray film.