This is a knockout-validated antibody summary, based on the publication "Alzheimer's Disease Risk Factor Pyk2 Mediates Amyloid-β-Induced Synaptic Dysfunction and Loss", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG2a
Company: Cell Signaling
Antibody: Pyk2
Catalog number: 3480
Summary: Mouse monoclonal IgG2a against a GST-Pyk2 fusion protein containing residues surrounding the carboxy-terminal of human Pyk2. Reacts with human and mouse. Suitable for western blot and immunoprecipitation.
Western blot
Brain cortex and hippocampus from WT and Pyk2−/− mice.
Blocking buffer for fluorescent Western blotting (Rockland MB-070-010) at room temperature for 1 h.
1:1,000 dilution overnight at 4°C.
Appropriate secondary antibodies (Odyssey donkey anti-mouse IRDye 680 or 800 conjugates, LI-COR Biosciences for 1 h at room temperature.
LI-COR Odyssey infrared imaging system.
Synaptic protein expression profile in cortex and hippocampus from 12-month-old WT and Pyk2−/− mice. Please see Figure 2g in the article [1].
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