This is a knockout-validated antibody summary, based on two publications "Post-transcriptional regulation of mouse neurogenesis by Pumilio proteins" [1] (western blot, see figure 2c in the article), and "An Important Role of Pumilio 1 in Regulating the Development of the Mammalian Female Germline", as cited below [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit monoclonal IgG
Company: Abcam
Antibody: Pumilio 1
Catalog number: ab92545
Summary: Rabbit monoclonal IgG against a synthetic peptide within human Pumilio 1( aa 150-250). Reacts with mouse, rat, and human. Suitable for western blot, immunocytochemistry /immunofluorescence, immunohistochemistry (paraffin) and flow cytometry. Unsuitable for immunoprecipitation.
Western blot
Wild-type and Pum1−/− mouse ovaries. Samples were prepared in M-Per Mammalian protein extraction reagent (Thermo Scientific) according to manufacturer’s instructions.
5% milk TBST solution for 30 min.
1:1,000 dilution overnight at 4 C.
1:4,000 dilution anti-rabbit conjugated horseradish peroxidase (Life Technologies) in 5% milk TBST for 1 h at room temperature.
Chemiluminescent signals were detected using Supersignal West Dura extended substrate (Thermo Scientific) and radiographic film.
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