This is a knockout-validated antibody summary, based on two publications "Post-transcriptional regulation of mouse neurogenesis by Pumilio proteins" [1] (western blot, see figure 2c in the article), and "An Important Role of Pumilio 1 in Regulating the Development of the Mammalian Female Germline", as cited below [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunoblot analysis showing PUM1 and PUM2 protein levels in wild-type mouse ovaries (+/+) and Pum1−/− ovaries (−/−) from 6- to 10-wk-old female mice. Glyceraldehyde phosphate dehydrogenase (GAPDH) used as loading control. From [2].

Rabbit monoclonal IgG

Company: Abcam

Antibody: Pumilio 1

Catalog number: ab92545

Summary: Rabbit monoclonal IgG against a synthetic peptide within human Pumilio 1( aa 150-250). Reacts with mouse, rat, and human. Suitable for western blot, immunocytochemistry /immunofluorescence, immunohistochemistry (paraffin) and flow cytometry. Unsuitable for immunoprecipitation.

Western blot

Wild-type and Pum1−/− mouse ovaries. Samples were prepared in M-Per Mammalian protein extraction reagent (Thermo Scientific) according to manufacturer’s instructions.

5% milk TBST solution for 30 min.

1:1,000 dilution overnight at 4 C.

1:4,000 dilution anti-rabbit conjugated horseradish peroxidase (Life Technologies) in 5% milk TBST for 1 h at room temperature.

Chemiluminescent signals were detected using Supersignal West Dura extended substrate (Thermo Scientific) and radiographic film.