Pten antibody | knockout validation | Abcam ab130224
DOI
//dx.doi.org/10.13070/ko.en.7.2051
Date
2017-01-22

This is a knockout-validated antibody summary, based on the publication "CRISPR-Cas9 delivery to hard-to-transfect cells via membrane deformation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Pten antibody | knockout validation | Abcam ab130224 figure 1
Figure 1. Immunofluorescence of Pten (in green) and pAkt (in red) in control (Cas9 ) and Pten-KO (sgPten) MCF7 cells. Scale bar: 10 µm. From [1].
Antibody information

Mouse monoclonal IgG1

Company: Abcam

Antibody: Pten

Catalog number: ab130224

Summary: Mouse monoclonal IgG1 against a purified recombinant fragment of human PTEN. Reacts with human and mouse.. Suitable for western blot, immunocytochemistry/immunofluorescence, flow cytometry and ELISA.

Validation Method

Immunofluorescence

Sample

Control and Pten-KO MCF7 cells. Cells grown overnight on coverslips were fixed in 4% paraformaldehyde and then permeabilized with 0.5% Triton X-100 plus 300 mM sucrose.

Secondary incubation

Alexa Fluor 488–conjugated goat anti-mouse (A-11001, Life Technologies).

Detection

Olympus FV1000 confocal microscope.

Clone note

The same clone (1B8) is sold as Abnova MAB10405; Abcam ab130224; Invitrogen MA5-15560.

References
  1. Han X, Liu Z, Jo M, Zhang K, Li Y, Zeng Z, et al. CRISPR-Cas9 delivery to hard-to-transfect cells via membrane deformation. Sci Adv. 2015;1:e1500454 pubmed publisher