Prr7 antibody | knockout validation | Exbio 11-617-C100
DOI
//dx.doi.org/10.13070/ko.en.7.2211
Date
2017-03-18

This is a knockout-validated antibody summary, based on the publication "Normal Development and Function of T Cells in Proline Rich 7 (Prr7) Deficient Mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Prr7 antibody | knockout validation | Exbio 11-617-C100 figure 1
Figure 1. Immunoblotting of Prr7 protein levels in whole brain extracts from Prr7+/+ and Prr7-/- mice. Blotting for tubulin served as a loading control. MW, molecular weight. From [1].
Antibody information

Mouse monoclonal IgG2a

Company: Exbio

Antibody: Prr7

Catalog number: 11-617-C100

Summary: Mouse monoclonal IgG2a against recombinant C-terminal half of the intracellular domain of human PRR7/TRAP3 (amino acids 126-253). Reacts with human, mouse and rat. Suitable for western blot and immunocytochemistry.

Validation Method

Western blot

Sample

Brain extracts from Prr7+/+ and Prr7-/- mice. Whole brain was homogenized and the crude membrane fraction (P2 fraction) isolated by centrifugation and lysed in RIPA buffer.

Secondary incubation

HRP-conjugated antibodies.

Detection

Chemiluminescence detection.

Clone note

The same clone (TRAP3/10) is sold as LifeSpan Biosciences LS-C112457; Abnova MAB6689; Invitrogen MA1-10448; Exbio 11-617-C100.

References
  1. Hrdinka M, Sudan K, Just S, Drobek A, Stepanek O, Schluter D, et al. Normal Development and Function of T Cells in Proline Rich 7 (Prr7) Deficient Mice. PLoS ONE. 2016;11:e0162863 pubmed publisher