This is a knockout-validated antibody summary, based on the publication "A therapeutic T cell receptor mimic antibody targets tumor-associated PRAME peptide/HLA-I antigens", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1
Company: Enzo Life Science
Antibody: Proteasome 20S β1i subunit
Catalog number: BML-PW8840-0025
Summary: Mouse monoclonal IgG1 against recombinant human β1i protein. Reacts with human and rat. Suitable for western blot and immunohistochemistry.
Western blot
Control and β1i-knock out SK-Mel5 melanoma cell lines. Total cell lysate was extracted using RIPA buffer.
5% milk for 1 hour block at room temperature.
Using the manufacturer’s s recommended dilution overnight at 4°C.
HRP-conjugated antibodies.
Immunoblot of the indicated proteins in control and β1i-knock out in SK-Mel5 melanoma cell lines using a CRISPR approach. Please see Figure 5a in the article [1].
The same clone (LMP2-13) is sold as LifeSpan Biosciences LS-C343796; Enzo Life Sciences BML-PW8840-0025.
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