This is a knockout-validated antibody summary, based on the publication "PSMA redirects cell survival signaling from the MAPK to the PI3K-AKT pathways to promote the progression of prostate cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Prostate Specific Membrane Antigen

Catalog number: 12815

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues in the extracellular domain of human prostate specific membrane antigen protein. Reacts with human, mouse and rat by western blotting and immunohistochemistry (paraffin).

Western blot

Control and PSMA-KO mouse TRAMP-C1 cell lines and human 22Rv1 cell lines. Cells were lysed in lysis buffer containing 50 mM tris (pH 7.4), 150 mM NaCl, 1% NP-40, plus the tyrosine phosphatase inhibitor Na3VO4 (1 mM), and the protease inhibitors phenylmethyl-sulfonyl fluoride (PMSF) (1 mM), pepstatin (1 mM), and aprotinin (1.5 mg/ml).

Tris-buffered saline, 5% bovine serum albumin (BSA) for 30 min.

For 12 hours.

For 30 min.

ECL Western blotting kit (Thermo Fisher Scientific).

Immunoblot analysis of PSMA in control and PSMA-KO mouse TRAMP-C1 cell lines and human 22Rv1 cell lines. Please see Figure 4a in the article [1].