This is a knockout-validated antibody summary, based on the publication "CRISPR-Cas9-based knockout of the prion protein and its effect on the proteome", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Identification of clones that exhibit loss of PrP expression by Western blot analyses of cellular lysates. From [1].

Mouse monoclonal IgG1k

Company: Bertin Pharma

Antibody: PrP

Catalog number: A03213

Summary: Mouse monoclonal IgG1k against proteinase K treated and non-denatured scrapie-associated fibrils from Syrian hamster infected brain (263K). Reacts with mouse, human and ovine by western blot and ELISA.

Western blot

WT and Prnp-KO N2a, C2C12 and NMuMG mouse cells. Cells were lysed with a buffer consisting of 50 mM Tris, pH 8.0, 150 mM NaCl, 1% NP-40, and Roche Complete Protease Inhibitor Cocktail.

Skim milk.

1∶5,000 dilution overnight at 4°C.

1∶10,000 dilution anti-mouse secondary antibody (170-6516; Bio-Rad, Mississauga, ON, Canada).

ECL reagent (RPN2106; GE Healthcare, Baie d'Urfe, QC, Canada). Signals were detected with either X-ray film or the Li-COR Odyssey Fc digital imaging system (NE, USA).