Pontin antibody | knockout validation | Sigma-Aldrich SAB4200194
DOI
//dx.doi.org/10.13070/ko.en.7.2241
Date
2017-04-08

This is a knockout-validated antibody summary, based on the publication "Pontin functions as an essential coactivator for Oct4-dependent lincRNA expression in mouse embryonic stem cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Pontin antibody | knockout validation | Sigma-Aldrich SAB4200194 figure 1
Figure 1. Immunoblot showing the depletion of Pontin in Pontinf/f; CreER ES cells at indicated days after OHT treatment. From [1].
Antibody information

Mouse monoclonal IgG

Company: Sigma-Aldrich

Antibody: Pontin

Catalog number: SAB4200194

Summary: Mouse monoclonal IgG against human Pontin fusion protein. Reacts with human, monkey and canine. Suitable for western blot, immunoprecipitation, immunofluorescence, and immunohistochemistry.

Validation Method

Western blot

Sample

Pontinf/f; CreER ES cells after OHT treatment.

Primary incubation

1/1,000 dilution at 4 °C overnight.

Secondary incubation

HRP-conjugated secondary antibodies (light chain-specific, Jackson Laboratory).

Detection

LAS 4000-mini system (Fujifilm).

References
  1. Boo K, Bhin J, Jeon Y, Kim J, Shin H, Park J, et al. Pontin functions as an essential coactivator for Oct4-dependent lincRNA expression in mouse embryonic stem cells. Nat Commun. 2015;6:6810 pubmed publisher