This is a knockout-validated antibody summary, based on the publication "DLX5, FGF8 and the Pin1 isomerase control ΔNp63α protein stability during limb development: a regulatory loop at the basis of the SHFM and EEC congenital malformations", as cited below [1], and "CDK5-dependent phosphorylation and nuclear translocation of TRIM59 promotes macroH2A1 ubiquitination and tumorigenicity" for western blot knockout validation (figure 3f) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunofluorescent staining with anti-Pin1 on sections of the embryonic hindlimbs of wild-type (A) or Dlx5;Dlx6-/- (B) embryos, and Pin1 KO embryos (C), at the age E11. Scale bar in A=20 m. The lack of staining in the Pin1-/- specimen indicates that the antibody is highly specific. From [1].

Mouse monoclonal IgG2a (kappa light chain)

Company: Santa Cruz

Antibody: Pin1

Catalog number: sc-46660

Summary: Mouse monoclonal IgG2a (kappa light chain) raised against amino acids 41-163 mapping at the C-terminus of Pin1 of human origin. Recommended for detection of Pin1 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Western blot Immunohistochemistry

Longitudinal sections of 12–15 μm of embryonic hindlimbs of WT, Dlx5;Dlx6 DKO mouse and Pin1 null mouse.

PBS with 1% BSA for 1 h at RT.

1:250 to 1:50 in PBS + 1% BSA, ON at 4°C.

Anti-mouse-Cy2 (Jackson ImmunoResearch) diluted 1:200, 1 h at RT.

Zeiss Observer-Z1 fluorescent microscope, equipped with Apotome system.