This is a knockout-validated antibody summary, based on the publication "The Topoisomerase 1 Inhibitor Austrobailignan-1 Isolated from Koelreuteria henryi Induces a G2/M-Phase Arrest and Cell Death Independently of p53 in Non-Small Cell Lung Cancer Cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. From Figure 5 in [1]. Western blot of lysates of human non-small cell lung cancer cell lines (A549, p53 positive; H1299, p53 null) probed with anti-phosph-p53 (Ser15) antibody. Actin serves as a loading control.

Rabbit polyclonal

Company: Cell Signaling Technology

Antibody: Phosho-p53 (Ser15)

Catalog number: 9284

Summary: Rabbit polyclonal antibody raised against a synthetic phosphor-peptide corresponding to residues surrounding Ser15 of human p53.

Supplier recommended for WB, IP and ChIP.

Peptide affinity-purified.

Reacts with phospho-p53 (Ser15) from human, mouse, rat and monkey. Does not detect other p53 phosphorylation sites.

Western blot

Lysates of human non-small cell lung cancer cell lines (A549, p53 positive; H1299, p53 null).

Anti-p53 antibody.

Chemiluminescence.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.