This is a knockout-validated antibody summary, based on the publication "FGF stimulation of the Erk1/2 signalling cascade triggers transition of pluripotent embryonic stem cells from self-renewal to lineage commitment", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit monoclonal

Company: Cell Signaling Technology

Antibody: Phospho-p44/42 MAPK (ERK1 and ERK2)

Catalog number: 4376 Clone 20G11

Summary: Rabbit monoclonal antibody raised against a synthetic phosphopeptide corresponding to the residues surrounding Thr202 and Tyr204 of human p44 MAPK. Recognises target protein singly phosphorylated at Thr202 and doubly phosphorylated at Thr202 and Tyr204 of p44 MAPK (residues Thr185 and Tyr187 of p42 MAPK). Supplier recommended for WB, IP and IHC. Reacts with human, mouse, rat, hamster, monkey, mink, Drosophila melanogaster, Saccharomyces cerevisiae, Zebrafish and pig phospho-p44/42 MAPK.

Western blot

Whole cell lysates of wild-type and Erk2 -/- (Erk2 = p42 MAPK) mouse embryonic stem cells.

1:1000 dilution.

Peroxidase conjugated anti-rabbit IgG (Sigma, A6154) at 1:3000 dilution.

Super Signal West (Pierce).

See figure 4 in the article [1].

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.