Phospho-Stat3 (Tyr705) antibody | knockout validation | Cell Signaling 9145

This is a knockout-validated antibody summary, based on the publication "Epidermal growth factor signaling protects from cholestatic liver injury and fibrosis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Phospho-Stat3 (Tyr705) antibody | knockout validation | Cell Signaling 9145 figure 1
Figure 1. Western blot analysis for tyrosine-705 phosphorylated pYSTAT3 (upper images) in STAT3flox/flox p19ARF−/− and STAT3Δhc p19ARF−/− hepatocytes after IL-6 treatment. The two pY-STAT3 bands represent STAT3α and STAT3ß isoforms. Expression of ß-Actin (lower images) was analyzed as loading control. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Phospho-Stat3 (Tyr705)

Catalog number: 9145

Summary: Rabbit monoclonal IgG against a synthetic phosphopeptide corresponding to residues surrounding Tyr705 of mouse Stat3. Reacts with human, mouse, rat and monkey. It is predicted to react based on 100% sequence homology with hamster, bovine, pig, and horse. Suitable for western blot, immunoprecipitation (IP), immunohistochemistry (paraffin and frozen), immunofluorescence/immunocytochemistry, flow cytometry and chromatin IP.

Validation Method

Western blot

Sample

STAT3flox/flox and STAT3Δhc mouse hepatocytes.

References
  1. Svinka J, Pflügler S, Mair M, Marschall H, Hengstler J, Stiedl P, et al. Epidermal growth factor signaling protects from cholestatic liver injury and fibrosis. J Mol Med (Berl). 2017;95:109-117 pubmed publisher