This is a knockout-validated antibody summary, based on the publication "A NIK-SIX Signalling Axis Controls Inflammation by Targeted Silencing of Non-Canonical NF-κB", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Cell Signaling
Antibody: Phospho-NF-κB p65 (Ser536)
Catalog number: 3033
Summary: Rabbit monoclonal IgG against a synthetic phosphopeptide corresponding to residues surrounding Ser536 of human NF-κB p65. Reacts with human, mouse, rat, hamster, monkey, and pig. It is predicted to react based on 100% sequence homology with dog. Suitable for western blot, immunoprecipitation, immunofluorescence/immunocytochemistry and flow cytometry.
Western blot
WT, RelA−/− or RelB−/− fibroblasts.
Western blot shows endogenous RelA and RelB expression in parental and RelA−/− or RelB−/− fibroblasts and response to stimulation by 24-hour application of TWEAK. Please see Extended Data Fig. 5b in the article [1].
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- product
- Cell Signaling Technology Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb
- Cell Signaling Technology Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Biotinylated)
- Cell Signaling Technology Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor® 488 Conjugate)
- Cell Signaling Technology Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor® 647 Conjugate)
- Cell Signaling Technology Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (PE Conjugate)