This is a knockout-validated antibody summary, based on the publication "EPHA2 blockade overcomes acquired resistance to EGFR kinase inhibitors in lung cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit monoclonal

Company: Cell Signaling Technology

Antibody: Phospho-EphA2 (Tyr588)

Catalog number: 12677

Summary: Rabbit monoclonal antibody raised against a synthetic phospho-peptide corresponding to residues surrounding Tyr588 of human EphA2. Supplier recommended for WB and IP. This antibody recognised EphA2 phosphorylated on Tyr588. It may cross-react with other over-expressed phosphor-tyrosine proteins. Reacts with human EphA2 (predicted to react with the corresponding mouse, rat and monkey proteins based on 100% sequence conservation).

Western blot

Lysates of lung tumor tissue from wild-type and EphA2 knockout mice.

TBS-T containing 5% milk powder.

Anti-phospho-EphA2 diluted 1:1000.

HRP-conjugated anti-rabbit IgG (Promega).

Clarity Western ECL reagent (BioRad).

Please see Figure S4 in [1].