This is a knockout-validated antibody summary, based on the publication "Renal peroxiredoxin 6 interacts with anion exchanger 1 and plays a novel role in pH homeostasis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG2a kappa

Company: Abcam

Antibody: Peroxiredoxin 6

Catalog number: ab16947

Summary: Mouse monoclonal IgG2a kappa against human recombinant full length human protein. Reacts with mouse, rat, and human. It is predicted to work with cow, ferret and orangutan. Suitable for western blot, immunocytochemistry/immunofluorescence, flow cytometry, and ELISA.

Western blot; IHC

Kidneys from Prdx6+/+ and Prdx6-/- mice. WB: Tissues were homogenized in 500 μl of cold homogenization buffer (10 mM Tris pH 7.4, 150 mM NaCl, 1 mM EGTA, 1 mM EDTA, 2 mM PMSF, and protease inhibitors). IHC: Mouse kidneys were fixed in 10% neutral buffered formalin, and paraffin-embedded.

IHC: 1:500 dilution overnight at 4°C.

IHC: 1:200 dilution fluorochrome conjugated secondary antibodies for 1hr at room temperature.

IHC: Zeiss LSM880 confocal microscope.

Kidney sections from Prdx6+/+ and Prdx6-/- mice were used to test the specificity of the anti PRDX6 antibodies Ab59543 (green) and Ab16947 (red) (A) Scale bars: 20 µm. Western blots with three samples each of Prdx6+/+ and Prdx6-/- total kidney lysate with a human kidney total (HKT) lysate as a control were probed with Ab59543 (B) and Ab16947 (C). Actin and tubulin were used as loading controls respectively. The sample preparations were carried out under reducing conditions. Please see Supplementary Figure 1 in the article [1].

The same clone (4A3) is sold as Abnova MAB0681; Abcam ab16947; Invitrogen LF-MA0018; LifeSpan Biosciences LS-B6255.