Peroxiredoxin 4 antibody | knockout validation | Abcam ab184167

This is a knockout-validated antibody summary, based on the publication "Peroxiredoxin 4 protects against ovarian ageing by ameliorating D-galactose-induced oxidative damage in mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Peroxiredoxin 4 antibody | knockout validation | Abcam ab184167 figure 1
Figure 1. Immunoblotting analysis of Prdx4 protein expression in the control and Prdx4−/− groups. The blot was probed with an anti-Prdx4 antibody. GAPDH was used as the internal control. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: Peroxiredoxin 4

Catalog number: ab184167

Summary: Rabbit monoclonal antibody against a synthetic peptide of Peroxiredoxin 4. Reacts with mouse and human. It is predicted to work with rat. Suitable for western blot and immunohistochemistry (paraffin).

Validation Method

Western blot

Sample

WT and Prdx4−/− ovarian tissues.

Blocking agent

5% BSA solution for 1 h.

Primary incubation

Diluted in blocking buffer overnight at 4 °C.

Secondary incubation

HRP-conjugated secondary antibodies.

Detection

Alpha Imager after development with Pierce ECL Western Blotting Substrate.

References
  1. Liang X, Yan Z, Ma W, Qian Y, Zou X, Cui Y, et al. Peroxiredoxin 4 protects against ovarian ageing by ameliorating D-galactose-induced oxidative damage in mice. Cell Death Dis. 2020;11:1053 pubmed publisher