This is a knockout-validated antibody summary, based on the publication "Single-cell dynamics of pannexin-1-facilitated programmed ATP loss during apoptosis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot analysis of PANX1 expression of wild-type and PANX1-KO HeLa cells. From [1].

Rabbit monoclonal IgG

Company: Cell Signalling

Antibody: Pannexin-1

Catalog number: 91137

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues near the amino terminus of human pannexin-1 protein. Reacts with human, mouse, and rat. Suitable for western blot and immunoprecipitation.

Western blot

WT and PANX1-KO HeLa cells.

Horseradish peroxidase (HRP)-labeled anti-rabbit IgG antibody (GE healthcare).

Chemi-Lumi One L reagent (Nacalai tesque) was used as a HRP substrate. A LAS4000 imager (GE healthcare) was used to detect the luminescence.