This is a knockout-validated antibody summary, based on the publication "Expression of protocadherin-γC4 protein in the rat brain", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG2b
Company: NeuroMab
Antibody: Pan-Gamma-protocadherin-A
Catalog number: 73-178
Summary: Mouse monoclonal IgG2b against a fusion protein amino acids 720-804 (variable cytoplasmic domain) of mouse gamma-protocadherin-A3. Reacts with mouse. Suitable for western blot and immunocytochemistry.
Immunocytochemistry
Mouse hippocampal cultures from E18 embryos.
5% normal goat serum (NGS)/0.3% Triton X-100/ 0.12M PB for 1 hour at RT,
1:15 dilution in for 2 days at 4°C in a mixture of primary antibodies raised in different species in 2%NGS/0.3% Triton X-100 in 0.12M PB at 4°C.
fluorophore conjugated secondary antibodies in 2%NGS/ 0.3%Triton in 0.12M PB for 1 hour at RT.
laser scanning confocal microscope.
Please see Figure 6b in the article [1].
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