This is a knockout-validated antibody summary, based on the publication "Pak2 is required for actin cytoskeleton remodeling, TCR signaling, and normal thymocyte development and maturation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. (Left panel) Western blot analysis of the thymus of Pak2F/F (WT), and Pak2F/F;Cd4-Cre (KO) mice. Anti-Erk1/2 antibody was used as loading control. Shown are representative of two independent experiments. (Right panel) Western blot analysis of thymi of Pak2F/F (WT), Pak2F/+;Lck-Cre (HET) and Pak2F/F;Lck-Cre (KO) mice. Anti-Erk1/2 antibody was used as loading control. From [1].

Rabbit polyclonal IgG.

Company: Origene

Antibody: Pak2

Catalog number: TA306346

Summary: Rabbit polyclonal IgG raised against a 14 amino acid peptide from near the amino terminus of human PAK2. Detects human, mouse, and rat protein in western blot, immunohistochemistry, and immunofluorescence.

Western blot

Thymus of Pak2F/F (WT), Pak2F/F;Cd4-Cre (KO), Pak2F/+;Lck-Cre (HET) and Pak2F/F;Lck-Cre (KO) mice.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.