This is a knockout-validated antibody summary, based on the publication "Loss of PTEN Facilitates Rosiglitazone-Mediated Enhancement of Platinum(IV) Complex LA-12-Induced Apoptosis in Colon Cancer Cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blotting of PTEN in HCT116 wt and PTEN-/- cells. β-actin served as loading control. From [1].

Mouse monoclonal IgG1

Company: Santa Cruz Biotechnology

Antibody: PTEN

Catalog number: sc-7974

Summary: Mouse monoclonal IgG1 raised against amino acids 388-400 mapping at the C-terminus of PTEN of human origin. Recommended for detection of PTEN of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Western blot

Human colon adenocarcinoma cell lines HCT116 wt (p53+/+, Bax+/-, Chk2+/+, PTEN+/+) and PTEN-/-. Cells were lysed in 1% SDS lysis buffer containing protease inhibitor cocktail (#P2714, Sigma–Aldrich) or Protease Inhibitor Cocktail Set I (#5391313, Calbiochem, Merck Millipore, Bedford, MA, USA), for phosphoprotein detection NaVO4 and NaF were added to the lysis solution.

5% non-fat milk or BSA for 1 h at RT.

1:500 dilution in 5% non-fat milk at 4°C overnight.

anti-mouse IgG (1:3,000, #NA931) (Amersham Biosciences, Piscataway, NJ, USA) for 1 h at RT.

Immobilon Western Chemiluminescent HRP Substrate (#WBKLS0500, Merck Millipore, Bedford, MA, USA).

The same clone (A2B1) is sold as Santa Cruz Biotechnology sc-7974, sc-7974 AC, sc-7974 HRP; BD Biosciences 560002, 560003, 559600.