This is a knockout-validated antibody summary, based on the publication "PTEN regulates cilia through Dishevelled", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit polyclonal
Company: Cell Signaling Technology
Antibody: PTEN
Catalog number: 9552S
Summary:
Rabbit polyclonal antibody raised against a synthetic peptide from the C-terminal region of human PTEN.
The antibody is peptide affinity purified.
Supplier recommended for WB and IP.
Reacts with human, mouse, rat, monkey and hamster PTEN.
Immunohistochemistry
Embryonic trachea from wild-type and conditional PTEN -/- mice.
PBS containing 3% BSA, 0.1% fish gelatin and 0.1% Tween.
1:300 in blocking buffer.
Leica microscope with Hamamatsu digital camera and Volocity (Perkin Elmer) software.
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.
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