This is a knockout-validated antibody summary, based on the publications "EGFR feedback-inhibition by Ran-binding protein 6 is disrupted in cancer", as cited below [1], "Targeted Deletion of PTEN in Kisspeptin Cells Results in Brain Region- and Sex-Specific Effects on Kisspeptin Expression and Gonadotropin Release" for immunohistochemistry knockout validation (see [2] figure 1b) and "Glioblastomas acquire myeloid-affiliated transcriptional programs via epigenetic immunoediting to elicit immune evasion" for western blot knockout validation (figure s1c) [3]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot of PTEN in whole-cell lysates of Ptenlox/lox and PtenΔ/Δ MEFs. From [1].

Mouse monoclonal IgG2b

Company: Cell Signaling

Antibody: PTEN

Catalog number: 9556

Summary: Mouse monoclonal IgG2b against a synthetic peptide corresponding to the carboxy-terminal sequence of human PTEN. Reacts with human, mouse, rat, hamster, and monkey. Suitable for western blot and immunoprecipitation.

Western blot

Control Ptenlox/lox and PtenΔ/Δ MEFs.

5% milk, 0.1% Tween, 10 mM Tris at pH 7.6, 100 mM NaCl.

1:1,000 dilution for 1 h at room temperature or overnight at 4 °C.

Anti-mouse HRP-conjugated antibodies (Jackson Immunoresearch)

Chemiluminescence ECL (Amersham).