This is a knockout-validated antibody summary, based on publications "ERF mutations reveal a balance of ETS factors controlling prostate oncogenesis", as cited below [1] for western blot, "Pten is necessary for the quiescence and maintenance of adult muscle stem cells" [2] for immunocytochemistry (supplement figure 1a in the article), "Pten Regulates Cardiomyocyte Differentiation by Modulating Non-CG Methylation via Dnmt3" for western blot knockout validation (figure 3e) [3], "Rapid interrogation of cancer cell of origin through CRISPR editing" for western blot knockout validation (figure 2a, 5b) [4], "Endometrial PTEN Deficiency Leads to SMAD2/3 Nuclear Translocation" for western blot knockout validation (figure 1a) [5], "Temporal evolution of cellular heterogeneity during the progression to advanced AR-negative prostate cancer" for western blot knockout validation (figure s2e) [6], and "Targeting HIC1/TGF-β axis-shaped prostate cancer microenvironment restrains its progression" for western blot knockout validation (figure s1b) [7]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot of the indicated proteins from prostate organoids derived from Pten+/+ and Pten−/− mice. Courtesy of the authors.

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: PTEN

Catalog number: 9188

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues in the carboxy-terminal sequence of human PTEN. Reacts with human, mouse, rat, monkey, and dog. It is predicted to react based on 100% sequence homology with chicken. Suitable for western blot, immunoprecipitation and immunohistochemistry (paraffin).

Western blot (this article), immunocytochemistry (see supplement figure 1a in [2] )

Prostate organoids derived from Pten+/+ and Pten−/− mice. Protein was extracted from cell lines using M-PER Reagent (Thermo Scientific).

LiCor system.