This is a knockout-validated antibody summary, based on the publication "Conditional deletion of the Pten gene in the mouse prostate induces prostatic intraepithelial neoplasms at early ages but a slow progression to prostate tumors", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Prostate tissues isolated from 4-week-old PtenloxP/loxP:Osr1-Cre and PtenloxP/loxP control mice were subjected to histological and immunohistochemical analyses. Representative images from different prostatic lobes, including AP, anterior; D/LP, dorsolateral, and VP, ventral prostate lobes, are shown. From [1].

Mouse monoclonal IgG

Company: Cascade Bioscience

Antibody: PTEN

Catalog number: 6H2.1

Summary: Mouse monoclonal IgG against an epitope corresponding to aa 304-403 of human Pten. Reacts with human and mouse protein and it is suitable for western blot, immunoprecipitation, immunocytochemistry, immunohistochemistry, immunofluorescence and ELISA.

Immunohistochemistry

Prostate tissues isolated from 4-week-old PtenloxP/loxP:Osr1-Cre and PtenloxP/loxP control mice. Mouse tissues were fixed in 10% neutral-buffered formalin and embedded in paraffin.

5% goat serum in PBS for 30 minutes at room temperature.

1∶100 dilution in 1% goat serum at 4°C overnight.

biotinylated anti-rabbit or anti-mouse secondary antibody (Vector Laboratories, BA-1000 or BA-9200) for 1 h, horseradish peroxidase streptavidin (Vector Laboratories, SA-5004) for 30 min at room temperature.

DAB kit (Vector Laboratories, SK-4100).

The same clone (6H2.1) is sold as EMD Millipore 04-035; Dako M3627; Cascade Bioscience ABM-2052.