PTBP1 antibody | knockout validation | Life Technologies 324800

This is a knockout-validated antibody summary, based on the publication "hnRNP I regulates neonatal immune adaptation and prevents colitis and colorectal cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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PTBP1 antibody | knockout validation | Life Technologies 324800 figure 1

Figure 1. Western blotting result shows efficient ablation of hnRNPI in the colonic epithelial cells of the knockout mice. hnRNPI protein levels from two knockout mice and two control sibling littermates are shown. From [1].

Antibody information

Mouse monoclonal IgG1

Company: Life Technologies

Antibody: PTBP1

Catalog number: 324800

Summary: Mouse monoclonal IgG1 against recombinant human PTB protein. Reacts with human, mouse, and rat. Suitable for western blot, immunocytochemistry/immunofluorescence, immunoprecipitation and flow cytometry.

Validation Method

Western blot

Sample

Isolated colonic epithelial cells from WT and PTBP1 (hnRNPI) knockout mice. Cells were homogenized in lysis buffer.

Secondary incubation

HRP-linked secondary antibodies.

Detection

ECL prime (G&E Healthcare Life Sciences).

References

Jin Z, Liang F, Yang J, Mei W. hnRNP I regulates neonatal immune adaptation and prevents colitis and colorectal cancer.. PLoS Genet. 2017;13:e1006672 pubmed publisher

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