PSTPIP1 antibody | knockout validation | Abnova H00009051-M02

This is a knockout-validated antibody summary, based on the publication "BAR Proteins PSTPIP1/2 Regulate Podosome Dynamics and the Resorption Activity of Osteoclasts", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

PSTPIP1 antibody | knockout validation | Abnova H00009051-M02 figure 1
Figure 1. Western blotting of PSTPIP1 and PSTPIP2 expression in control and PSTPIP1-KO murine osteoclasts. From [1].
Antibody information

Mouse monoclonal IgG2b Kappa

Company: Abnova

Antibody: PSTPIP1

Catalog number: H00009051-M02

Summary: Mouse monoclonal antibody raised against a full-length recombinant PSTPIP1. Reacts with human and mouse. Suitable for western blot and ELISA.

Validation Method

Western blot

Sample

Control and PSTPIP1-KO murine osteoclasts.

Primary incubation

1:500 dilution.

Secondary incubation

1:5,000 dilution horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Jackson ImmunoResearch Laboratories, Suffolk, UK).

Clone note

The same clone (1D5) is sold as LifeSpan Biosciences LS-C139170; Abnova H00009051-M02; Sigma-Aldrich SAB1404662.

References
  1. Sztacho M, Segeletz S, Sanchez-Fernandez M, Czupalla C, Niehage C, Hoflack B. BAR Proteins PSTPIP1/2 Regulate Podosome Dynamics and the Resorption Activity of Osteoclasts. PLoS ONE. 2016;11:e0164829 pubmed publisher