This is a knockout-validated antibody summary, based on the publication "Nephron-specific deletion of the prorenin receptor causes a urine concentration defect", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit polyclonal IgG

Company: Abcam

Antibody: PRR

Catalog number: ab40790

Summary: Rabbit polyclonal IgG against a synthetic peptide derived from within residues 300 to the C-terminus of human ATP6IP2. Reacts with human and rat. Predicted to work with mouse, chicken, and cow. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin and frozen), flow cytometry.

Western blot | Immunohistochemistry

Control and PRR KO mice kidney. Renal inner medulla was dissected and homogenized in ice-cold sucrose buffer (10 mM triethanolamine and 250 mM sucrose, pH 7.6) with PMSF (100 μg/ml), leupeptin (10 μM), and Complete Protease Inhibitors (Roche, Pleasanton, CA). IHC: Kidneys were fixed overnight in 10% formaldehyde and embedded in paraffin, and 4-μm sections were obtained.

IHC: 1% BSA in PBS for 1 h.

IHC: 1:50 dilution overnight.

IHC: 1:50 dilution anti-rabbit Alexa fluor 488 for 60 min.

WB: Advance ECL system (GE Healthcare, Piscataway, NJ).

IHC: Nikon FXA epifluorescence microscope.

Please see Figure 2 and 3 in the article [1].

The same clone (20C11B11B11) is sold as Abcam ab14713; Invitrogen 459100.