This is a knockout-validated antibody summary, based on the publication "Pharmacological inhibition of PRMT7 links arginine monomethylation to the cellular stress response", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. PRMT7 (P7) knockout (KO) or knockdown (KD) reduces HSP70 methylation in various cell lines. 94A, 21B—HCT116 CRISPR PRMT7 KO clones; P parental C2C12; C-C2C12 expressing control guide RNA; 32 C2C12 CRISPR clone expressing PRMT7 catalytic mutant (delY35,A35S); 4,57,74—C2C12 CRISPR Prmt7 KO clones. PRMT7 was knocked down in HEK293T and MCF7 cells using siRNA, Con control, P7KD PRMT7 knockdown. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: PRMT7

Catalog number: ab179822

Summary: Rabbit monoclonal IgG against a synthetic peptide within human PRMT7 (aa 1-100, cysteine residue, N-terminal). Reacts with mouse, rat, and human. Suitable for western blot. Unsuitable for flow cytometry, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin) or immunoprecipitation.

Western blot

WT and PRMT7-KO HCT116, C2C12 and MEF cells.

5% milk in PBS for 1 h.

1:1,000 dilution in 5% BSA in PBST (0.1% Tween20 PBS) overnight at 4 °C.

1:5,000 goat-anti-rabbit IgG-IR800 (#926-32211, LiCor) in Odyssey Blocking Buffer (LiCor) for 1 h at RT .

Odyssey scanner (LiCor) at 800 and 700 nm.