PRMT6 antibody | knockout validation | Santa Cruz sc-271744

This is a knockout-validated antibody summary, based on the publication "PRMT5-mediated arginine methylation activates AKT kinase to govern tumorigenesis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

PRMT6 antibody | knockout validation | Santa Cruz sc-271744 figure 1
Figure 1. Immunoblot (IB) analysis of whole cell lysates (WCL) derived from MCF7 cells infected with PRMTs-targeting lentiCRISPR virus (sg-1 and sg-2) and GFP-targeting lentiCRISPR virus as a negative control (con). From [1].
Antibody information

Mouse monoclonal IgG1 κ

Company: Santa Cruz

Antibody: PRMT6

Catalog number: sc-271744

Summary: Mouse monoclonal antibody against amino acids 181-310 mapping within an internal region of human PRMT6. Recommended for detection of PRMT6 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Validation Method

Western blot

Sample

Control and PRMT6 KO MCF7 cells.

Blocking agent

5% non-fat milk in TBST.

Primary incubation

1:2,000 dilution in blocking buffer.

Secondary incubation

1:3,000 dilution peroxidase-conjugated anti-mouse secondary antibody (A4416, Sigma).

References
  1. Yin S, Liu L, Brobbey C, PALANISAMY V, Ball L, Olsen S, et al. PRMT5-mediated arginine methylation activates AKT kinase to govern tumorigenesis. Nat Commun. 2021;12:3444 pubmed publisher