This is a knockout-validated antibody summary, based on the publications "AMPK-SKP2-CARM1 signalling cascade in transcriptional regulation of autophagy" [1] (discussed below) and and "Targeting the IRE1α/XBP1s pathway suppresses CARM1-expressing ovarian cancer" for western blot knockout validation (figure 2a) [2] "AMPK-SKP2-CARM1 signalling cascade in transcriptional regulation of autophagy" [1] (see the extended figure 1d in the article), and "Targeting the IRE1α/XBP1s pathway suppresses CARM1-expressing ovarian cancer" for western blot knockout validation (figure 2a) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Cell Signaling
Antibody: PRMT4/CARM1
Catalog number: 3379
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to the amino terminus of the human PRMT4/CARM1 protein. Reacts with human, mouse, rat, and monkey. Suitable for western blot, immunoprecipitation and immunofluorescence/immunocytochemistry.
Western blot
Wild-type, Carm1 knockout and knock-in mouse embryonic fibroblasts (MEFs).
Immunoblot analysis of wild-type (WT), Carm1 knockout (KO) or knock-in (KI) MEFs. Please see Figure 1c in the article [1].
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