PRMT4/CARM1 antibody | knockout validation | Cell Signaling 12495

This is a knockout-validated antibody summary, based on the publication "PRMT5-mediated arginine methylation activates AKT kinase to govern tumorigenesis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

PRMT4/CARM1 antibody | knockout validation | Cell Signaling 12495 figure 1
Figure 1. Immunoblot (IB) analysis of whole cell lysates (WCL) derived from MCF7 cells infected with PRMTs-targeting lentiCRISPR virus (sg-1 and sg-2) and GFP-targeting lentiCRISPR virus as a negative control (con). From [1].
Antibody information

Mouse monoclonal IgG1

Company: Cell Signaling

Antibody: PRMT4/CARM1

Catalog number: 12495

Summary: Mouse monoclonal antibody against a recombinant protein specific to the human CARM1 protein. Reacts with human, mouse, rat and monkey. Suitable for western blot, immunoprecipitation and immunofluorescence/immunocytochemistry.

Validation Method

Western blot

Sample

Control and PRMT4 KO MCF7 cells.

Blocking agent

5% non-fat milk in TBST.

Primary incubation

1:1,000 dilution in blocking buffer.

Secondary incubation

1:3,000 dilution peroxidase-conjugated anti-mouse secondary antibody (A4416, Sigma).

References
  1. Yin S, Liu L, Brobbey C, PALANISAMY V, Ball L, Olsen S, et al. PRMT5-mediated arginine methylation activates AKT kinase to govern tumorigenesis. Nat Commun. 2021;12:3444 pubmed publisher